QPCR(Polymerase Chain Reaction) gives you a choice of quantitative methods for gene expression.The NB988 Real-time PCR is very useful and important for life science research and curative evaluation, and it is innovative and pioneering products in Lab. for the prevention, diagnosis, evaluation and monitoring of diseases.
Specifications
|
Profiles |
1-9 |
|
Repetitions |
1-99 |
|
Steps |
1-9 |
|
Set times |
1-9999sec |
|
Profile linkage |
Yes |
|
Reagents |
FAM,SYBR GREEN I,JOE,HEX,VIC (various fluorescence PCR reagents , such as TaqMan probe, etc |
|
Sample capacity |
48 × 0.2ml or 36 × 0.2ml |
|
Sample handing |
Thick-walled 0.2ml PCR tubes |
|
Sample volume |
20-100μl |
|
Wavelength of exciting fluorescence |
495nm，535nm |
|
Wavelength of fluorescence detection |
525nm，555nm |
|
Monochromator device |
Filter,made in USA |
|
Photo detector |
PMT(Photomultipier tube), made in Japan |
|
Heating/cooling device |
TE Peltier(Thermoelectric Peltier Effect module),made in Japan |
|
Quantitative accuracy |
R≥0.990, CV≤3% |
|
Heating rate |
3℃/sec |
|
Cooling rate |
2℃/sec |
|
Thermal range |
4.0℃-99.0℃ |
|
Thermal accuracy |
±0.5℃ |
|
Thermal uniformity |
±0.5℃ |
|
System interfaces |
RS-232C serial interface, bi-directional |
|
Computer operating system |
Windows 2000/XP |
|
Power supply |
AC220V/110V, 50Hz/60Hz |
|
Power |
<800W |
|
Operating environment |
Ambient Temperature 5℃-40℃ Relative Humidity 30%-70% |
|
Weight |
26kg |
|
Dimensions(W×D× H) |
500mm × 400mm × 350 mm |
Heating & cooling unit
Applying the advantages of thermoelectric (TE, Peltier Effect) module makes temperature change rapidly and uniform. Hot-lid design permits oil-free operation.
Optical unit
An array of LED collimates to corresponding wells.High-light and long-lived LED excitation light source does not need maintenance or preheating.
PMT fluorescence detection offers high sensitivity, broad dynamic range and low crosstalk.
An array of detecting coaxial optical fibers focus on each well’s bottom ensure minimal light loss.
Operation system and Application software
Application software interface is friendly windows style, Monitoring real-time amplification plots and data analysis during a PCR run.
Supply software package of medical case and database management and plot display.
Quantification
After quantitative analysis for the standard sample, the instrument will get a standard curve of Ct value related to copy number.
Comparing the Ct value of unknown sample with standard curve, the instrument can calculate the original copy number of unknown sample by quantitative analysis. NB988 has a wide linear detecting range, which can detect 101-1010 DNA copies/ml.
