Description:
Gene gun technology (particle bombardment) is a all-new gene-delivery technology, also a physical method, in which the genetic material or other substances attached to a high-speed microbomb are shot directly into the cells, so it is a late gene-transferred technology internationally. The pneumatic Gas gene gun can make a wide range of cell types instantly stable and highly-efficient transformation. The gene gun has a special rupture which generates a gas shock ware. The degree of Helium-pressure that drives the carrier film is determined by the different rupture films used in the process. Within a proper atmospheric pressure the different rupture films penetrate the DNA-coated microcariers into the target cells at the bottom of the bombardment chamber. The gene gun is applicable in gene-transformation of plants and animals, cell cultures, embryos, bacteria and small animals. It has fast, simple, safe and efficient features. The device purchased by Genetics, Neuroscience Research Institutes under the Chinese Academy of Sciences, Fudan University, Zhejiang University, Shandong Agricultural University, and other relevant units .Articles concerming the product were published，such as：Zhejiang University gournal in the magazine 《Tea》2002.28（3），Plant transformation technology by using the gene gun and its application prospects in tea Cultivation; Fudan University Genetic institute published abroad, “Transformation and Functional Expression of the rFCA-RRM2 Gene in Rice” in《Plant Biology》.
Characteristics：a unique circular bombardment transformation room, removable for easy cleansing and disinfection, low cost.
Standard Configuration: host, vaccuum pumps, breakage film, carrier film, stainless steel net, tungsten powder.
Main technical parameters and application
|
Experimental conditions |
In vitro, from the body, plants (in vivo) |
|
Target region |
（big）40cm2 |
|
Pressure range |
3-12MPa |
|
Target Type |
Animals: cell and organ culture Plants: The smaller of the whole plants, cell culture, explant, yeast, bacteria and other micro-organelles, chloroplast, mitochondria |
|
Voltage |
220V/110V |
|
Gas |
Helium or nitrogen ( helium is more otimized) |
|
Metal Particle |
Φ0.6-1.6μm，（Common Φ1μm） |
